Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective

Table 2 Primers used for amplification of specific regions of the virulence genes of E. coli

Target gene	Primer code	Sequence (5’→3’)	Amplified products (bp)	References
eae	EAE1	F: AAACAGGTGAAACTGTTGCC	490	[32]
eae	EAE2	R: CTCTGCAGATTAACCTCTGC		[32]
stx2	EVT1	F: CAACACTGGATGATCTCAGC	350	[33]
stx2	EVT2	R: CCCCCTCAACTGCTAATA		[33]
hlyA	EHCF F	F: ACGATGTGGTTTATTCTGGA	167	[34]
hlyA	EHCF R	R: CTTCACGTCACCATACATAT		[34]
bfpA	BFPAF	F: AATGGTGCTTGCGCTTGCTGC	324	[35]
bfpA	BFPAR	R: GCCGCTTTATCCAACCTGGTA		[35]
st	ST1	F: TTTATT TCT GTA TTG TCT T	294	[36]
st	ST2	R: GCAGGATTACAACACAATTC		[36]
lt	LT1	F: GGCGACAGATTATACCGTGC	696	[37]
lt	LT2	R: CCGAATTCTGTTATATATGTC		[37]

Key: eae = intimin, stx2 = shiga toxin2, hlyA = hemolysin gene, bfpA = bundle forming pilli, st = heat-stable enterotoxin, lt = heat-labile enterotoxin, F = forward primer, R = reverse primer, bp = base pair

ISSN: 2524-4655