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Table 3 Preparation of the PCR mixture for each targeted gene

From: Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective

PCR component

Amount of PCR component (µl)

stx2 and eae

st and lt

bfpA and hlyA

Buffer

2.5

2.5

2.5

MgCl2

1.5

2

1.5

Nuclease free water

14.5

13

15

dNTP

1

1

1

Forward primer

1

1

0.5

Reverse primer

1

1

0.5

Taq DNA polymerase

0.5

0.5

0.5

DNA template

3

4

3.5

Total

25

25

25

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One Health Outlook

ISSN: 2524-4655